Illumina/TruSeq Stranded mRNA Library

Illumina/TruSeq Stranded mRNA Library Prep Kit for NeoPrep (16 samples, 24 indexes)/NP-202-1001/1 Ea

Illumina/TruSeq Stranded mRNA Library Prep Kit for NeoPrep (16 samples, 24 indexes)/NP-202-1001/1 Ea

Product Highlights:
The TruSeq Stranded mRNA Library Prep Kit for NeoPrep offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It enables robust interrogation of both standard and low-quality samples.

Simplified and seamless workflow solution – Generate libraries with minimal hands-on time
Unparalleled performance and reproducibility – Interrogate gene expression with TruSeq Stranded mRNA coverage and quality, and reduced user variABIlity
Low input requirement – Begin library prep with as little as 25 ng total RNA
Precise and Accurate
Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Cost-Efficient
Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.

NeoPrep System enhances TruSeq Stranded mRNA performance
The NeoPrep System enhances TruSeq Stranded mRNA performance by providing high-quality reproducIBLe results, even with low input amounts of RNA. Digital microfluidics technology precisely manipulates droplets that perform the library prep workflow, within the tightly controlled environment of the NeoPrep library card.

A simple, intuitive workflow delivers 16 libraries, eliminating most manual steps, and reducing hands-on time from ~4.5 hours to just 30 minutes. In addition, digital microfluidics requires less RNA input, enabling excellent performance from as little as 25 ng of total RNA.

Specifications:
Assay Time ~10.5 hours
Hands-On Time ~30 minutes
Input Quantity 25 to 100 ng total RNA from species with polyA tails
Content Specifications Captures the coding transcriptome with strand information
Mechanism of Action Oligo-dT beads capture polyA tails
Multiplexing Up to 24-plex pooling with additional NeoPrep runs
Sample Throughput Up to 16 samples per NeoPrep library card
System Compatibility Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000
Species Category Other,Mammalian,Bovine,Mouse,Human,Rat
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Technology Sequencing
Method mRNA Sequencing
Automation CapABIlity NeoPrep Digital Microfluidics